Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Morphological changes of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p) after 24 h treatment.

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Apoptosis of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Cell cycle analysis of A549, A431, HL60, and IMR90 cell lines untreated (a, e, i, m) and, treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Cell Cycle Assay, Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Caspase-3 expression of A549, A431, HL60, and IMR90 cell-lines un-treated (a, e, i, m), treated with IC 50 concentration of the standard drug (b, f, j, n), SNP-B (c, g, k, o), and SNP-C (d, h, l, p).

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Concentration Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Percentage Cell Viability of A549, A431, HL60 and, IMR90 Cell-Lines Treated with Various Concentrations of SNP-B and SNP-C a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Control

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Apoptotic Study (i.e., Annexin V/PI Expression Study) of SNP-B and SNP-C against A549, A431, HL60, and IMR90 Cell-Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Cell Cycle Analysis of SNP-B and SNP-C against A549, A431 HL60, and IMR90 Cell Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Cell Cycle Assay

Journal: ACS Omega

Article Title: Cytotoxic and Apoptotic Effects of Chemogenic and Biogenic Nano-sulfur on Human Carcinoma Cells: A Comparative Study

doi: 10.1021/acsomega.1c04047

Figure Lengend Snippet: Caspase-3 Expression Study of SNP-B and SNP-C against A549, A431, HL60, and IMR90 Cell Lines a

Article Snippet: Human epidermoid carcinoma (A431), human promyelocytic leukemia (HL60) ,human lung carcinoma (A549), and human lung fibroblast (IMR90) cell lines were procured from the National Centre for Cell Science (NCCS), Pune, India.

Techniques: Expressing, Fluorescence

Journal: Biomedicines

Article Title: The Thioredoxin System of Mammalian Cells and Its Modulators

doi: 10.3390/biomedicines10071757

Figure Lengend Snippet: Cytotoxicity of TrxR inhibitors.

Article Snippet: 3. Organoselenium compounds , 3.1. Organo- selenium compounds 3.2. Phenylarsenic oxide derivatives (dithiarsanes) , 1,2-(5,5′-Dimethoxy(bis-1,2-benzisoselenazol-3(2 H )-one))ethane 2-(4-Aminophenyl)-1,3,2-dithiarsenane (PAO−PDT) , , 1.64 0.6 , U-87MG ATCC HL-60 , [ ] [ ] .

Techniques: Analogues

Journal: bioRxiv

Article Title: Citrullination of TDP-43 is a key post-translation modification associated with structural and functional changes and progressive pathology in TDP-43 mouse models and human proteinopathies

doi: 10.1101/2025.02.28.639952

Figure Lengend Snippet: a, The enzymatic reaction mediated by PADs converts arginine to citrulline. b, c, Western blot images of PAD2 and PAD4-mediated citrullination of H3 (b) and TDP-43 (c). In (c) arrows indicate the observed shift in TDP-43 molecular weight. d, Coomassie staining of PAD2 (bands 1, 4), PAD4 (bands 2, 6), unmodified TDP-43 (band 3), PAD2-mediated citR TDP-43 (band 5), and PAD4-mediated citR TDP-43 (band 7). e, Bar diagrams showing the MW shift (kDa) of TDP-43 protein following citrullination. f, Schematic representation of arginine epitopes positioned in the human TDP-43 protein sequence. Position of the 11 citrullinated arginine epitopes are indicated in red. g, Six out of eleven arginine epitopes susceptible to citrullination laid within common RX(X)R (red box) or RXG/RGGG (green box) motifs in TDP-43 sequence. h, MS/MS spectrum showing b- and y-ion coverage of modified citR83 peptide and extracted ion chromatograms (XICs) showed abundance and retention time of unmodified R83 vs. citR83 with PAD2 and PAD4 treatment (intact peptide monoisotopic m/z (+2) 719.8457, (+3) 480.2329). i, Retention time peaks for TDP-43 peptides surrounding the unmodified or modified R83 epitope and base peak m/z undergoing methionine (Met85) oxidation, citrullination or both showed reliable time separation.

Article Snippet: PAD2 and PAD4 primary antibodies were purchased from Proteintech, US (#12110-1-AP and 17373-1-AP) and diluted at 1:10000 vs. 1:1000, respectively.

Techniques: Western Blot, Molecular Weight, Staining, Sequencing, Tandem Mass Spectroscopy, Modification

Journal: bioRxiv

Article Title: Citrullination of TDP-43 is a key post-translation modification associated with structural and functional changes and progressive pathology in TDP-43 mouse models and human proteinopathies

doi: 10.1101/2025.02.28.639952

Figure Lengend Snippet: Spectra showing b- and y-ion coverage of citrullinated peptides, as well as XICs retention times for unmodified and citrullinated peptides treated with PAD2 and PAD4, corresponding to a - c, citR165 (intact peptide monoisotopic m/z (+2) 665.8214), d - f, citR191(intact peptide monoisotopic m/z (+2) 672.8469), g - i, citR268/272 (intact peptide monoisotopic m/z (+2) 728.3560), j - l, citR293 (intact peptide monoisotopic m/z (+2) 638.29).

Article Snippet: PAD2 and PAD4 primary antibodies were purchased from Proteintech, US (#12110-1-AP and 17373-1-AP) and diluted at 1:10000 vs. 1:1000, respectively.

Techniques:

Journal: bioRxiv

Article Title: Citrullination of TDP-43 is a key post-translation modification associated with structural and functional changes and progressive pathology in TDP-43 mouse models and human proteinopathies

doi: 10.1101/2025.02.28.639952

Figure Lengend Snippet: a, Schematic representation of seven polyclonal antibodies raised against the citrullinated arginine epitopes. Epitope positions within each TDP-43 functional domain is indicated. b, Antibody specificity was determined by western blot via PAD4-mediated citrullinated TDP-43 protein probed with citR83 (NLS), citR165 and citR191 (RRM1, RRM2), citR268/272, citR275 (CTD) antibodies. c, Immunohistochemical images of TAR4/4 and non-Tg littermates cortical tissue labeled against citR TDP-43 antibody panel. Increased neuronal expression of citR83, citR165, citR191, citR268/272 and citR275 in TAR4/4 tissue compared to Non-Tg tissue. Arrows indicate the citR TDP-43 morphologies observed with the citR268/272 and citR275 antibodies raised against TDP-43 C-terminal domain (arrows). Insets represent magnified neuronal citR TDP-43 morphological properties recognized from each antibody. d, Images of cortical tissue incubated with secondary antibody only - “no-primary” control. n = 3, scale bar is 50µm and 200 µm.

Article Snippet: PAD2 and PAD4 primary antibodies were purchased from Proteintech, US (#12110-1-AP and 17373-1-AP) and diluted at 1:10000 vs. 1:1000, respectively.

Techniques: Functional Assay, Western Blot, Immunohistochemical staining, Labeling, Expressing, Incubation, Control

Journal: bioRxiv

Article Title: Citrullination of TDP-43 is a key post-translation modification associated with structural and functional changes and progressive pathology in TDP-43 mouse models and human proteinopathies

doi: 10.1101/2025.02.28.639952

Figure Lengend Snippet: a, Immunohistochemical images of Non-Tg, TAR4 and TAR4/4 cortex labeled with PAD2 and PAD4 antibodies. b, Fold change of PAD2 and PAD4 (% area mean of the values ± SEM) normalized to the Non-Tg control. One-way or Two-way ANOVA, followed by Tukey’s post hoc multiple comparisons tests, n = 5, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. c, Schematic overview of the TAR TDP-43 mouse model and the fractionation of cortical tissue into RIPA-soluble and (7 M) Urea-soluble fractions. d, The expression levels of the human TDP-43 in the TAR model. e, Cortical RIPA soluble and f, Urea soluble fraction analyzed by Western blotting and probed for citR TDP-43 antibody panel (citR83, 165, 191, 268/272, 275), pTDP-43 409/410 and total human TDP-43 protein. g, Quantification of citR TDP-43 43 kDa protein levels, proteolytic fragments (17, 25 & 35 kDa) and intermediate high molecular species (72, 98 & 120k Da) normalized to GAPDH in RIPA and h, Urea fraction normalized to the total protein loaded signal. Data represent the mean of the values ± SEM; One-way ANOVA, followed by Tukey’s or Šidák post hoc multiple comparisons tests, n = 5, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: PAD2 and PAD4 primary antibodies were purchased from Proteintech, US (#12110-1-AP and 17373-1-AP) and diluted at 1:10000 vs. 1:1000, respectively.

Techniques: Immunohistochemical staining, Labeling, Control, Fractionation, Expressing, Western Blot

Journal: Immunology

Article Title: Functional exhaustion of CD4 + T cells induced by co‐stimulatory signals from myeloid leukaemia cells

doi: 10.1111/imm.12665

Figure Lengend Snippet: Up‐regulation of surface molecules related to activation and/or exhaustion on CD4 + T cells co‐cultured with different myeloid leukaemia cell lines for 96 hr under continuous stimuli conditions

Article Snippet: Human myeloid leukaemia cell lines, KG‐1, Kasumi‐1, HL‐60, U937 and THP‐1 were either obtained from the American Type Culture Collection (ATCC, LGC Promochem, Rockville, MD) or received as kind gifts.

Techniques: Activation Assay, Expressing

Journal: The Journal of Biological Chemistry

Article Title: Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation *

doi: 10.1074/jbc.M115.702787

Figure Lengend Snippet: Step-by-step protocol applied to screen the library of bioactive compounds at The Broad Institute

Article Snippet: Human promyelocytic leukemia HL60 cells (Sigma) differentiated into neutrophil-like cells by all- trans -retinoic acid were used as the HTS-compatible source of Nox2 model system for screening Nox2 inhibitors ( 25 , 26 ).

Techniques: Cell Culture, Concentration Assay, Inhibition, Fluorescence

Journal: The Journal of Biological Chemistry

Article Title: Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation *

doi: 10.1074/jbc.M115.702787

Figure Lengend Snippet: Positive hits obtained during screening of the library of bioactive compounds using three probes as follows: hydropropidine (50 μ m ) in the presence of DNA (0.1 mg/ml) as a probe for Ȱ, and coumarin boronic acid (100 μ m ) or Amplex Red (50 μ m ) in the presence of HRP (0.1 units/ml) as probes for H 2 O 2 Differentiated HL60 cells were stimulated with PMA (1 μ m ) to induce Nox2 activity in HBSS supplemented with HEPES buffer (25 m m ) and dtpa (0.1 m m ).

Article Snippet: Human promyelocytic leukemia HL60 cells (Sigma) differentiated into neutrophil-like cells by all- trans -retinoic acid were used as the HTS-compatible source of Nox2 model system for screening Nox2 inhibitors ( 25 , 26 ).

Techniques: Activity Assay

Journal: The Journal of Biological Chemistry

Article Title: Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation *

doi: 10.1074/jbc.M115.702787

Figure Lengend Snippet: IC 50 values for selected positive hits, determined by monitoring the effect of the compounds on the rate of oxidation of coumarin boronic acid (100 μ m ) as a probe for H 2 O 2 Differentiated HL60 cells were stimulated with PMA (1 μ m ) to induce Nox2 activity in HBSS supplemented with HEPES buffer (25 m m ) and dtpa (0.1 m m ) and the extent of probe oxidation was monitored in 384-well plates using fluorescence plate reader.

Article Snippet: Human promyelocytic leukemia HL60 cells (Sigma) differentiated into neutrophil-like cells by all- trans -retinoic acid were used as the HTS-compatible source of Nox2 model system for screening Nox2 inhibitors ( 25 , 26 ).

Techniques: Activity Assay, Fluorescence